Resource Sharing

Welcome to the resource sharing section of our site. The following Centers have listed the available resources they have to share and those they need. Click on the links for details.


JDRF-BRI Center for Translational Research

Technologies / Resources to Share:

  • Tetramers – $3.50 service fee (requester provides peptide solution)
  • SNP genotyping – $250-$500, depending on number of samples
  • Immunohistochemistry – $2.50-$35.00, depending on service
  • Mouse model (HLA-DR4 GAD Tcr) – $TBD

Restrictions:

  • MTA: Tetramers and mouse model (with ACUC approval)
  • IRB
  • Informed Consent for Collaboration

Cost:

See above

Contact Person(s):

Barbara Droker, 206-223-8812, bdroker@benaroyaresearch.org

Technologies / Resources Desired:

None at this time


The JDRF Collaborative Center for Cellular Therapy

Technologies / Resources to Share:

  • Cell expansion technologies
  • Regulatory Expertise
  • RMS360 quality mAbs

Restrictions:

Access to RMS360 mAbs will require an agreement with Becton-Dickenson.

Cost:

Regulatory support will require reimbursement for time spent by the regulatory staff at University of Pennsylvania. Cost for mAbs will depend on agreements with BD

Contact Person(s):

Jeffrey Bluestone – 415-514-1683

Technologies / Resources Desired:

  • Virus technologies for T cell receptor and other immune gene packaging to optimize transduction into expanded Tregs
  • Technologies (tetramers, etc) that enhance antigen-specific expansion of Tregs in humans
  • Imaging technologies that allow tracking of expanded Tregs in humans

JDRF Center for Developing Immune Therapies for Type 1 Diabetes at Yale University

Technologies / Resources to Share:

We will be producing immune deficient mice in which murine genes, that we believe are essential for growth and development of an immune system, have been replaced with human genes. This will occur in an iterative manner beginning with the knockins described in the proposal. As these mice are prepared and characterized by our laboratory, we may be able to make the strains available to other investigators.

Likewise, the hu Ins transgenic mouse, described in Project 4 may be made available to other investigators once the strain has been produced and characterized.

We are planning to obtain serum samples and cells from patients with type 1 diabetes. This material, which is de-identified, may be made available to other investigators.

Restrictions:

  • MTA: This will be needed for receipt of mice.
  • IRB: The human samples will be de-identified. IRB approval is not needed for receipt of the samples.
  • Informed Consent for Collaboration: N/A

Cost:

Not yet determined.

Contact Person(s):

Elizabeth Eynon (203) 785-5384
Li Wen (203) 737-5071

Technologies / Resources Desired:

Table 1 Mouse Strains Supplied by the Core

Strain Name Background Availability How Supplied Project Use
NODscid/IL2R³c-/-RIP-hGAD65-A NOD To be bred for project and then investigator to take over Provide as breeding pairs Project 4, possibly 2
NODscid. IL2R³c-/-hIns Tg NOD To be bred for project and then investigator to take over Provide as breeding pairs Project 4
NODscid. IL2R³c-/­mIns1-/-mIns2-/- hIns Tg NOD To be bred for project and then investigator to take over Provide as breeding pairs Project 4/td>
B6 DQ8mII­RIPB7/hReg B6 To be bred for project and then investigator to take over Provide as breeding pairs Project 3
B6 DQ8DR4mII­RIPB7 B6/td> Available Provide as breeding pairs Project 3, pilot 1
B6 DR4mII-RIPB7 Balb/c x 129 Available Provide as breeding pairs Project 2
Rag2-/-, IL2R³c-/-(Rag/³) (HHLS) Balb/c x 129 Limited Provide as breeding pairs Project 1
hTPO, Rag/³ (HHLS) Balb/c x 129 Limited Provide as breeding pairs Project 1
hIL3/GM-CSF, Rag/³ (HHLS) Balb/c x 129 Limited Provide as breeding pairs Project 1
HLA-A2.1 Rag/³ (HHLS) Balb/c x 129 In progress Provide as breeding pairs Project 1, 5
HLA-A2, DR4, Rag/³ (HHLS) Balb/c x 129 In progress Provide as breeding pairs Project 1, 2, 5
hTPO/hIL3/GM­CSF/HLA­A2,DR4,DQ8, Rag/³ (HHLS) Balb/c x 129 This project Provide as breeding pairs Project 1, 2, 4, 5

JDRF/WT Diabetes and Inflammation Laboratory (DIL)

Technologies / Resources to Share:

Research protocols in molecular biology and genetics; informatics including T1Dbase.org, showing the latest genetics results; genotyping; DNA samples, plasma and sequencing, serum samples from T1D cases, and controls; EBV cell lines from T1D cases; statistical methods; data access and distribution; flow cytometry, human blood cell profiling and protocols; autoantibody data.

Restrictions:

  • MTA: Possibly required for genotyping, sample and data sharing
  • IRB: Additional uses of clinical material would require revisions of IRB approval; Informed Consent for Collaboration

Cost:

Depends on quantity, frequency

Contact Person(s):

PI: Professor John Todd
Administrator: Mrs. Judy Brown
Clinical Resources: Ms. Sarah Nutland
Data Services: Mr. Neil Walker
DNA and Cell Resources: Ms Helen Stevens
Genome Informatics: Mr. Olly Burren
Mouse Genetics and Immunology: Professor Linda Wicker
Statistics: Mr. David Clayton
Systems Management: Mr. Vin Everett

Technologies / Resources Desired:

Plasma serum and DNA samples from unaffected siblings and T1D cases. Polychromatic flow cytometry data from profiling human blood samples. Gene expression profiling of purified human blood cell sub populations. Access to ultra high throughput sequencing technology.


Cord Blood Therapies for Type 1 Diabetes

Technologies / Resources to Share:
Human umbilical cord blood
Consultation and potential access to RMS360 core for cell therapies
Consultation and potential access to unique strains of humanized mice
***Consultation and potential access to human pancreas and related tissues
***Consultation and potential access to a Core dedicated to crystallography and molecular modeling

***Note: These are not Center resources, but rather JDRF (or other) funded resources that are present at UF and potentially open to Center investigators.

Restrictions:

  • MTA:
  • IRB: 04/20/2009
  • Informed Consent for Collaboration:

Cost:

$1,362,866

Contact Person(s):

Mark A. Atkinson, Ph.D.
Kimberly Young

Technologies / Resources Desired:

Genetic screening for non-MHC loci for type 1 diabetes.


The BDC JDRF Autoimmunity Prevention Center

Technologies / Resources to Share:

Diabetes-related autoantibody analyses:

  • IA2 (IA2ic, ICA512bdc)
  • Phogrin
  • PTPrN (hybrid molecule of IA2c and phogrin)
  • GAD2 (GAD65)
  • Insulin
  • ZnT8 (N-term, C-term Arg325, C-term Trp325, C-term Gln325, Nterm/ C-term Trp325 fusion, C-term Arg325/ C-term Trp325 fusion)
  • Transglutaminase (Celiac disease)
  • 21-hydroxylase (Addison’s disease)
  • Interferon alpha (APS-1)

Measurement of:

  • ISLET: GAD65, IA-2ic, ICA512bdc, insulin, and ZnT8 autoantibodies
  • ADRENAL: 21-hydroxylase
  • Celiac: Transglutaminase
  • Anti-interferon (diagnosis of APS-I syndrome)
  • Development of novel fluid phase radioimmunoassays utilizing in vitro transcription and translation of autoantigens or other molecules.
  • Teach groups to utilize fluid phase radioimmunoassay and/or to develop their own radioimmunoassay.
  • Sharing expertise in antibody epitope mapping

Elispot Assays:

Available for both mice (IFN8) and humans (IFN8, IL-10)

Molecular Core:

  • Vectors, cell lines and expertise in the construction of retrogenic mice
  • DNA sequencing analyses including 454 high throughput used for TCR diversity analysis
  • PCR analyses for Speed congenic animal breeding programs
  • QRTPCR for gene expression profiling
  • Custom genotyping for common SNPs

Antibodies:

Antibodies developed to ß-cell proteins that are not commercially available. Eg. IGRP, ZnT8

Animals:

  • NODbdc
  • NODbdc scid

Clinical Samples:

  • New onset human diabetic sera
  • Genotyped (DR/DQ) sera for controls
  • Access to monozygotic twin data, DNA and sera
  • Access to DNA and sera from longitudinal studies of diabetes incidence (DAISY, TEDDY)

Restrictions:

  • Age 3 and above
  • MTA:
  • IRB: #01-384
  • Informed consent for collaboration: Yes

Cost:

  • Procurement of blood sample $ 20
  • Participant compensation $ 20
  • PBMC isolation $ 30
  • Sample storage in Liquid Nitrogen $ 30
  • Shipping sample to collaborator $150

Contact Person(s):

Administration: Kathryn Gray, Kathryn.Gray@uchsc.edu, 303-724-6836

Core A: Danny.Zipris@uchsc.edu

Core C: Peter.Gottlieb@uchsc.edu; Rebecca.Wagner@uchsc.edu

Project 1: George.Eisenbarth@uchsc.edu; Liping.Yu@uchsc.edu

Project 2: John.Hutton@uchsc.edu; Janet.Wenzlau@uchsc.edu

Project 3: Howard.Davidson@uchsc.edu

Technologies / Resources Desired:

PBMCs from T1D subjects and controls, contact Howard Davidson @ Howard.Davidson@uchsc.edu, 303-724-6852


JDRF Center on Immunological Tolerance in Type 1 Diabetes at Harvard Medical School

Technologies / Resources to Share:

See our Center page.

Restrictions:

  • MTA: Required for all recipients for certain mouse lines. Certain mouse lines cannot be freely provided to for-profit entities.
  • IRB: may be necessary for human samples.
  • Informed Consent for Collaboration:

Cost:

Please see http://www.citdh.org/

Contact Person(s):

Core A:
for existing mouse lines – jose.pagan@joslin.harvard.edu
for generation of new lines – john.stockton@joslin.harvard.edu

Core B:
britta.svoren@joslin.harvard.edu

Technologies / Resources Desired:

Umbilical chord samples from T1D patients and controls


Diabetes Genes, Autoimmunity & Prevention (D-GAP)

Technologies / Resources to Share:

Core A: Samples from FDRs; serum, DNA, possibly cells

Core C: Genotyping technology and optimal SNP determination for risk alleles

Projects 1 and 2: ELISPOTs for autoreactive T cells (Th1, Th2, Th17); HLA class I autoantigenic epitopes; class I tetramer technology, CD8 cloning, cytotoxicity assays

Project 3: polychromatic flow cytometry protocols and statistical analyses; immunoassay protocols

P&F Project: Human Treg analysis (functional and phenotypic)

Restrictions:

  • MTA: Possibly required for genotyping
  • IRB: Additional uses of clinical material would require revisions of IRB approval
  • Informed Consent for Collaboration:

Cost:

Depends upon quantity, frequency.

Contact Person(s):

Core A: Professor David Dunger

Core B: Professor Polly Bingley

Core C: Professor John Todd

Projects 1,2: Professor Mark Peakman

Project 3: Professors Linda Wicker and John Todd

P&F Project: Dr Tim Tree.

Technologies / Resources Desired:

HLA class II tetramers for proinsulin specific epitopes restricted by HLA-DR4 and HLA-DR3

Serum and DNA samples from other studies of unaffected siblings, or high risk new borne/other cohorts with other information where possible (eg autoAb status) eg Finnish newborn cohorts, DPT-1, ENDIT, TEDDY, TRIALNET Natural History Study etc